| Title | Estimating the variation in S phase duration from flow cytometric histograms. |
| Authors | Sara Larsson, Tobias Rydén, Ulla Holst, Stina Oredsson, Maria C Johansson |
| Alternative Location | http://www.ncbi.nlm.nih.gov..., Restricted Access |
| Alternative Location | http://dx.doi.org/10.1016/j..., Restricted Access |
| Publication | Mathematical biosciences |
| Year | 2008 |
| Volume | 213 |
| Issue | 1 |
| Pages | 40 - 49 |
| Document type | Article |
| Status | Published |
| Quality controlled | Yes |
| Language | eng |
| Publisher | Elsevier |
| Abstract English | A stochastic model for interpreting BrdUrd DNA FCM-derived data is proposed. The model is based on branching processes and describes the progression of the DNA distribution of BrdUrd-labelled cells through the cell cycle. With the main focus on estimating the S phase duration and its variation, the DNA replication rate is modelled by a piecewise linear function, while assuming a gamma distribution for the S phase duration. Estimation of model parameters was carried out using maximum likelihood for data from two different cell lines. The results provided quite a good fit to the data, suggesting that stochastic models may be a valuable tool for analysing this kind of data. |
| Keywords | Branching process, Cell cycle progression, S phase duration, Flow cytometry, |
| ISBN/ISSN/Other | ISSN: 0025-5564 |
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